Mycobacterium indicus pranii supernatant induces apoptotic cell death in mouse peritoneal macrophages in vitro

PLoS One. 2011 Feb 11;6(2):e17093. doi: 10.1371/journal.pone.0017093.

Abstract

Mycobacterium indicus pranii (MIP), also known as Mw, is a saprophytic, non-pathogenic strain of Mycobacterium and is commercially available as a heat-killed vaccine for leprosy and recently tuberculosis (TB) as part of MDT. In this study we provide evidence that cell-free supernatant collected from original MIP suspension induces rapid and enhanced apoptosis in mouse peritoneal macrophages in vitro. It is demonstrated that the MIP cell-free supernatant induced apoptosis is mitochondria-mediated and caspase independent and involves mitochondrial translocation of Bax and subsequent release of AIF and cytochrome c from the mitochondria. Experiments with pharmacological inhibitors suggest a possible role of PKC in mitochondria-mediated apoptosis of macrophages.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Active Transport, Cell Nucleus / drug effects
  • Animals
  • Apoptosis / drug effects*
  • Apoptosis Inducing Factor / metabolism
  • Bacterial Vaccines / adverse effects
  • Caspase 3 / metabolism
  • Cell Nucleus / drug effects
  • Cell Nucleus / metabolism
  • Culture Media, Conditioned / metabolism
  • Culture Media, Conditioned / pharmacology
  • Cytochromes c / metabolism
  • Down-Regulation / drug effects
  • Female
  • Injections
  • Lipopolysaccharides / pharmacology
  • Macrophages, Peritoneal / cytology*
  • Macrophages, Peritoneal / drug effects*
  • Macrophages, Peritoneal / metabolism
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mitochondria / drug effects
  • Mitochondria / metabolism
  • Mycobacterium / cytology*
  • Mycobacterium / immunology
  • Poly (ADP-Ribose) Polymerase-1
  • Poly(ADP-ribose) Polymerases / metabolism
  • Protein Kinase C-delta / antagonists & inhibitors
  • Protein Kinase C-delta / metabolism
  • Protein Kinase Inhibitors / pharmacology
  • Proteolysis / drug effects
  • Proteomics
  • bcl-2-Associated X Protein / metabolism

Substances

  • Apoptosis Inducing Factor
  • Bacterial Vaccines
  • Culture Media, Conditioned
  • Lipopolysaccharides
  • Pdcd8 protein, mouse
  • Protein Kinase Inhibitors
  • bcl-2-Associated X Protein
  • Cytochromes c
  • Parp1 protein, mouse
  • Poly (ADP-Ribose) Polymerase-1
  • Poly(ADP-ribose) Polymerases
  • Protein Kinase C-delta
  • Caspase 3