[Polysorbate-80 modified neurotoxin nanoparticle with its transport and cytotoxicity against blood-brain barrier]

Yao Xue Xue Bao. 2010 Oct;45(10):1312-6.
[Article in Chinese]


This study was aimed at the transport across blood-brain barrier (BBB) of polysorbate-80 modified neurotoxin loaded polybutylcyanoacrylate nanoparticle (P-80-NT-NP) and its cytotoxicity. An in vitro model of BBB using rat brain microvascular endothelial cells (rBMECs) was established. The cytotoxicity of P-80-NT-NP was measured by the MTT assays, where neurotoxin (NT), nanoparticle (NP), neurotoxin nanoparticle (NT-NP) as control, and the permeability of P-80-NT-NP was determined by using of Millicell insert coculture with rBMECs and fluorescence spectrophotometry. MTT results showed that NT, NP, NT-NP and P-80-NT-NP were avirulent to rBMECs when the concentration of NT was lower than 200 ng x mL(-1). But the cytotoxicity of NP, NT-NP and P-80-NT-NP would be augmented accordingly as concentration increased (P < 0.01), causing obvious reductions of cell survival rate, with no significant difference between them (P > 0.05). When the concentration of NT was 150 ng x mL(-1), the permeability on rBMECs of P-80-NT-NP and NT-NP were both significantly higher than that of NT (P < 0.01), and the permeability of P-80-NT-NP was greater than that of NT-NP (P < 0.05). In conclusion, polysorbate-80 modified neurotoxin nanoparticles can transport across the BBB, while concentration of NT is greater than 200 ng x mL(-1), P-80-NT-NP has a little cytotoxicity against rBMECs.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport
  • Blood-Brain Barrier*
  • Brain / blood supply
  • Capillary Permeability
  • Cell Survival / drug effects
  • Cells, Cultured
  • Drug Carriers
  • Electric Impedance
  • Enbucrilate / chemistry
  • Enbucrilate / toxicity
  • Endothelial Cells / cytology*
  • Endothelial Cells / metabolism
  • Female
  • Male
  • Nanoparticles
  • Neurotoxins / administration & dosage*
  • Neurotoxins / pharmacokinetics*
  • Particle Size
  • Polysorbates / chemistry*
  • Polysorbates / toxicity
  • Rats
  • Rats, Sprague-Dawley


  • Drug Carriers
  • Neurotoxins
  • Polysorbates
  • Enbucrilate