Isolation and characterization of a fungus Aspergillus sp. strain F-3 capable of degrading alkali lignin

Biodegradation. 2011 Sep;22(5):1017-27. doi: 10.1007/s10532-011-9460-6. Epub 2011 Feb 25.


A fungus strain F-3 was selected from fungal strains isolated from forest soil in Dalian of China. It was identified as one Aspergillus sp. stain F-3 with its morphologic, cultural characteristics and high homology to the genus of rDNA sequence. The budges or thickened node-like structures are peculiar structures of hyphae of the strain. The fungus degraded 65% of alkali lignin (2,000 mg l(-1)) after day 8 of incubation at 30°C at pH 7. The removal of colority was up to 100% at 8 days. The biodegradation of lignin by Aspergillus sp. F-3 favored initial pH 7.0. Excess acid or alkali conditions were not propitious to lignin decomposing. Addition of ammonium L: -tartrate or glucose delayed or repressed biodegradation activities. During lignin degradation, manganese peroxidase (28.2 U l(-1)) and laccase (3.5 U l(-1))activities were detected after day 7 of incubation. GC-MS analysis of biodegraded products showed strain F-3 could convert alkali lignin into small molecules or other utilizable products. Strain F-3 may co-culture with white rot fungus and decompose alkali lignin effectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus / enzymology
  • Aspergillus / genetics
  • Aspergillus / isolation & purification*
  • Aspergillus / metabolism*
  • Biodegradation, Environmental
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Hydrogen-Ion Concentration
  • Laccase / genetics
  • Laccase / metabolism
  • Lignin / chemistry
  • Lignin / metabolism*
  • Peroxidases / genetics
  • Peroxidases / metabolism
  • Soil Microbiology


  • Fungal Proteins
  • Lignin
  • Laccase
  • Peroxidases
  • manganese peroxidase