1H, 13C, and 15N resonance assignments for the CTD-interacting domain of Nrd1 bound to Ser5-phosphorylated CTD of RNA polymerase II

Karel Kubíček; Josef Pasulka; Hana Černá; Frank Löhr; Richard Štefl

doi:10.1007/s12104-011-9300-y

1H, 13C, and 15N resonance assignments for the CTD-interacting domain of Nrd1 bound to Ser5-phosphorylated CTD of RNA polymerase II

Biomol NMR Assign. 2011 Oct;5(2):203-5. doi: 10.1007/s12104-011-9300-y. Epub 2011 Feb 26.

Authors

Karel Kubíček¹, Josef Pasulka, Hana Černá, Frank Löhr, Richard Štefl

Affiliation

¹ National Centre for Biomolecular Research, Central European Institute of Technology, Faculty of Science, Masaryk University, 62500, Brno, Czech Republic. karelk@chemi.muni.cz

PMID: 21350922
DOI: 10.1007/s12104-011-9300-y

Abstract

In this article, we report the resonance assignment of CTD-interacting domain (CID) of pre-mRNA down-regulation (Nrd)1 bound to Ser5-phosphorylated CTD (pSer5) of RNA Polymerase II. The presented assignment of backbone and side-chain resonances of the Nrd1 CID proton, carbon and nitrogen nuclei will allow studies of the structure and interaction of CID with carboxy-terminal domain (CTD) of the RNA polymerase II.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Binding Sites
Down-Regulation
Isotopes / chemistry
Nuclear Magnetic Resonance, Biomolecular*
Peptides / chemistry
Peptides / metabolism
Phosphorylation
Protein Binding
Protein Structure, Tertiary
RNA Polymerase II / chemistry*
RNA Polymerase II / metabolism
RNA-Binding Proteins / chemistry*
RNA-Binding Proteins / metabolism
Saccharomyces cerevisiae Proteins / chemistry*
Saccharomyces cerevisiae Proteins / metabolism
Serine / chemistry
Serine / metabolism

Substances

Isotopes
NRD1 protein, S cerevisiae
Peptides
RNA-Binding Proteins
Saccharomyces cerevisiae Proteins
Serine
RNA Polymerase II