[Genetic polymorphism of XRCC1 associated with susceptibility of chromosomal damage in workers exposed by 1,3-butadiene]

Wei Sheng Yan Jiu. 2010 Nov;39(6):659-63.
[Article in Chinese]

Abstract

Objective: To explore the relationship between genetic polymorphism of XRCC1 194, 280, 399 and susceptibility of chromosomal damage induced by 1,3-butadiene (BD).

Methods: 138 workers occupationally exposed to BD and 41 normal individuals were involved. Cytokinesis-block micronucleus (CB-MN) assay was used to detect chromosome damage in peripheral lymphocyte. PCR-RFLP technique was applied to detect polymorphisms in XRCC1 194, 280 and 399.

Results: The MN frequencies (3.39 +/- 2.42) per thousand of workers exposed to BD were more higher than those of the non-exposed groups (1.48 +/- 1.26) per thousand (P < 0.01). Workers receiving mere exposures had higher MN values than those of lower-exposed workers: frequency ratios (FR) = 1.30 (95% CI 1.14 - 1.53, P < 0.05). Workers with XRCC1 194 Arg/Trp genotype had more susceptibility for chromosome damage, FR = 1.13 (95% CI 1.07 - 1.27). Workers with XRCC1 280 Arg/His genotype had more susceptibility for chromosome damage, FR = 1.67 (95% CI 1.10 - 2.42). XRCC1 399 Arg/Gln and combined group of Arg/Gln and Gln/Gln genotype carrier had higher MN frequency, FR = 1.26 (95% CI 1.03 - 1.53) and 1.24 (95% CI 1.03 - 1.49) respectively. The haplotypes CAG/TGG could associate with the decreased frequencies of total micronucleus (P < 0.05).

Conclusion: Genotype of XRCC1 could associated with the chromosome damage induced by BD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Butadienes / toxicity*
  • Chemical Industry
  • China
  • DNA Damage
  • DNA-Binding Proteins / genetics*
  • Female
  • Gene-Environment Interaction*
  • Genotype
  • Humans
  • Male
  • Micronuclei, Chromosome-Defective*
  • Occupational Exposure / adverse effects
  • Polymorphism, Genetic*
  • X-ray Repair Cross Complementing Protein 1

Substances

  • Butadienes
  • DNA-Binding Proteins
  • X-ray Repair Cross Complementing Protein 1
  • XRCC1 protein, human
  • 1,3-butadiene