A set of multiplex PCRs for genotypic detection of extended-spectrum β-lactamases, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases

Int J Antimicrob Agents. 2011 Apr;37(4):356-9. doi: 10.1016/j.ijantimicag.2011.01.005. Epub 2011 Feb 25.


Worldwide, resistance of Gram-negative micro-organisms to third-generation cephalosporins and carbapenems owing to β-lactamases is an increasing problem. Although the CTX-M, TEM and SHV extended-spectrum β-lactamases (ESBLs) are most widely disseminated, other β-lactamase families have also recently emerged, such as plasmid-mediated AmpC β-lactamases and carbapenemases. Here we describe a new set of multiplex polymerase chain reactions (PCRs) with one amplification protocol enabling detection of 25 prevalent β-lactamase families, including ESBLs, carbapenemases, plasmid-mediated AmpC β-lactamases and OXA β-lactamases.

MeSH terms

  • Bacterial Proteins / metabolism*
  • Base Sequence
  • DNA Primers
  • Genotype
  • Plasmids*
  • Polymerase Chain Reaction / methods*
  • beta-Lactamases / metabolism*


  • Bacterial Proteins
  • DNA Primers
  • beta-Lactamases
  • carbapenemase