In situ detection of warfarin using time-correlated single-photon counting

Annika M Rosengren; Björn C G Karlsson; Inga Näslund; Per Ola Andersson; Ian A Nicholls

doi:10.1016/j.bbrc.2011.02.103

In situ detection of warfarin using time-correlated single-photon counting

Biochem Biophys Res Commun. 2011 Apr 1;407(1):60-2. doi: 10.1016/j.bbrc.2011.02.103. Epub 2011 Feb 24.

Authors

Annika M Rosengren¹, Björn C G Karlsson, Inga Näslund, Per Ola Andersson, Ian A Nicholls

Affiliation

¹ Bioorganic and Biophysical Chemistry Laboratory, School of Natural Sciences, Linnaeus University, SE-391 82 Kalmar, Sweden.

PMID: 21354105
DOI: 10.1016/j.bbrc.2011.02.103

Abstract

Here we report on a novel method for the direct in situ measurement of specific isomeric forms of the anticoagulant warfarin using time correlated single-photon counting (TCSPC) spectroscopy in conjunction with synthetic Sudlow I binding site receptors. The method is highly robust over the clinically significant concentration range, and demonstrates the potential of the binding site mimics in conjunction with the spectroscopic strategy employed here for the determination of this important pharmaceutical in clinical or even environmental samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anticoagulants / analysis*
Anticoagulants / chemistry
Isomerism
Molecular Imprinting
Photons*
Spectrum Analysis / methods
Time Factors
Warfarin / analysis*
Warfarin / chemistry

Substances

Anticoagulants
Warfarin