Investigating wound healing, tyrosinase inhibitory and antioxidant activities of the ethanol extracts of Salvia cryptantha and Salvia cyanescens using in vivo and in vitro experimental models

J Ethnopharmacol. 2011 Apr 26;135(1):71-7. doi: 10.1016/j.jep.2011.02.022. Epub 2011 Feb 24.


Ethnopharmacological relevance: Salvia L. species are widely used against wounds and skin infections in Turkish folk medicine.

Aim of the study: The aim of the present study is to evaluate wound healing activity of the ethanol (EtOH) extracts of Salvia cryptantha and Salvia cyanescens.

Materials and methods: For the assessment of wound healing activity linear incision and circular excision wound models were employed on rats and mice. The wound healing effect was comparatively evaluated with the standard skin ointment Madecassol(®). Inhibition of tyrosinase, a key enzyme in skin aging, was achieved using ELISA microplate reader. Antioxidant activity was evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and superoxide radical scavenger effect, ferrous ion-chelating ability, and ferric-reducing antioxidant power (FRAP) tests.

Results and conclusions: The EtOH extract of Salvia cryptantha treated groups of animals showed 56.5% contraction, whereas the reference drug Madecassol(®) showed 100% contraction. On the other hand, the same extract on linear incision wound model demonstrated a significant increase (33.2%) in wound tensile strength as compared to other groups. The results of histopathological examination maintained the upshot of linear incision and circular excision wound models as well. These findings specify that Salvia cryptantha for wound healing activity can be appealed further phytochemical estimation for spotting its active components.

MeSH terms

  • Animals
  • Anti-Infective Agents / pharmacology
  • Antioxidants / pharmacology
  • Antioxidants / therapeutic use*
  • Biphenyl Compounds / metabolism
  • Disease Models, Animal
  • Male
  • Medicine, Traditional
  • Mice
  • Monophenol Monooxygenase / antagonists & inhibitors*
  • Phytotherapy*
  • Picrates / metabolism
  • Plant Components, Aerial
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use
  • Rats
  • Rats, Sprague-Dawley
  • Salvia*
  • Skin / drug effects*
  • Skin / injuries
  • Skin / pathology
  • Tensile Strength / drug effects
  • Triterpenes / pharmacology
  • Turkey
  • Wound Healing / drug effects*
  • Wounds, Stab / drug therapy*
  • Wounds, Stab / pathology


  • Anti-Infective Agents
  • Antioxidants
  • Biphenyl Compounds
  • Picrates
  • Plant Extracts
  • Triterpenes
  • 1,1-diphenyl-2-picrylhydrazyl
  • Monophenol Monooxygenase
  • asiaticoside