[Suppressive effect of gypenosides on murine leukemia L1210 cell lines]

Zhong Yao Cai. 2010 Oct;33(10):1588-92.
[Article in Chinese]

Abstract

Objective: To investigate the suppressive effect of gypenosides (Gyp) on murine leukemia L1210 cells.

Methods: The growth inhabitation of murine leukemia L1210 cell was detected by MT assay. The production of reactive oxygen species and the change of mitochondrial membrane potential were detected by flow cytometry. The change of nuclear and DNA damage of murine leukemia L1210 cells were detected by DAPI staining and single cell gel electrophoresis.

Results: Gyp (100-500 microg/mL) inhibited the growth of murine leukemia L1210 cells. The concentration of Gyp (350 microg/mL) treated murine leukemia L1210 cells at different time points, the mitochondrial membrane potential decrease obviously. L1210 cells were treated with Gyp (350 microg/mL) for 4 h, the highest production of reactive oxygen species was induced. DNA damage were detected after Gyp (350 microg/mL) treated for 4, 12, 24 h. The change of nuclear was treated by Gyp (350 microg/mL) with time-dependent.

Conclusion: Gypenosides has effects on cell viability, induce reactive oxygen species and decreases mitochondrial membrane potential, and can induce morphological changes and DNA damage.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antineoplastic Agents, Phytogenic / administration & dosage
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Cell Line, Tumor
  • Cell Proliferation / drug effects*
  • Cell Survival / drug effects
  • DNA Damage
  • Flow Cytometry
  • Gynostemma / chemistry*
  • Indoles
  • Leukemia L1210 / pathology*
  • Membrane Potential, Mitochondrial / drug effects
  • Mice
  • Plant Extracts / administration & dosage
  • Plant Extracts / pharmacology
  • Reactive Oxygen Species / metabolism
  • Time Factors

Substances

  • Antineoplastic Agents, Phytogenic
  • Indoles
  • Plant Extracts
  • Reactive Oxygen Species
  • gypenoside
  • DAPI