Objective: To study the protective effects of the combination of sandostatin(SS) and gardenia jasminoides ellis (GJE) on pancreatic mitochondria injury in severe acute pancreatitis (SAP) rats.
Methods: SAP rat model was induced by retrograde injection of 15 g/L sodium deoxycholate into biliary tract. The rats were randomly divided into five groups: SAP group, Sham group, SS group, GJE group, as well as SS and GJE combination group. The changes of Superoxide dismutase(SOD), succinic dehydrogenase (SDH), Na+ -K+ -ATPase, Ca2+ - Mg2+ -ATPase, malondialdehyde (MDA), membrance fluidity of mitochondria in pancreatic tissue were measured.
Results: Compared to Sham group, the activities of SOD, SDH, Na+ -K+ -ATPase and Ca2+ -Mg2+ -ATPase in mitochondria were obviously decreased in SAP group, and MDA and microviscosity (eta) were markedly increased (P < 0.01), which were associated with the increase of serum amylase level (P < 0.01). The above-mentioned indexes of GJE group, SS group and combination group were obviously ameliorated in comparison with SAP group (P < 0.01), the improvement in combination group was the best (P < 0.05 or P < 0.01). The correlation analysis indicated that MDA was negatively correlated with SOD, membrance fluidity, as well as the activities of Na+ -K+ -ATPase and Ca2+ -Mg2- -ATPase (r = -0.857, P < 0.01; r = -0.960, P < 0.01; r = -0.966, P < 0.01; r = -0.926, P < 0.01).
Conclusion: The combination of sandostatin and gardenia jasminoides ellis can protect pancreatic mitochondria injury in severe acute pancreatitis.