EMMPRIN (CD147) regulation of MMP-9 in bronchial epithelial cells in COPD

Respirology. 2011 May;16(4):705-12. doi: 10.1111/j.1440-1843.2011.01960.x.


Background and objective: Extracellular matrix metalloproteinase inducer (EMMPRIN or CD147) induces the production of matrix metalloproteinases (MMP) such as MMP-9, which plays an important role in COPD. We determined its cellular origin and role in MMP-9 production in COPD.

Methods: Bronchial biopsies, alveolar macrophages (AM) and blood monocytes (BM) from patients with COPD, healthy smokers and non-smokers, and bronchial epithelial cells (EC) from surgically resected airways from patients with COPD were stimulated with LPS or CRP in the presence and absence of an anti-EMMPRIN blocking antibody. EMMPRIN in BAL, plasma, conditioned media and cell lysates was quantified and immunohistochemical localization of EMMPRIN was determined in bronchial biopsies. MMP-9 activity and mRNA was also determined.

Results: EMMPRIN levels in BAL fluid were higher in patients with COPD compared with non-smokers and smokers. There was greater EMMPRIN expression in EC from patients with COPD compared with smokers and non-smokers. EC secreted and expressed more EMMPRIN protein than BM and AM. Blocking EMMPRIN decreased MMP-9 activity in supernatant of EC, but not in those from AM and BM, and decreased MMP-9 mRNA expression in EC.

Conclusions: The increased EMMPRIN expression in COPD is reflected by an increased release from bronchial EC, which are one of the main source of EMMPRIN. EMMPRIN regulates MMP-9 expression in COPD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Antibodies, Blocking / pharmacology
  • Basigin / metabolism*
  • Biopsy
  • Bronchi / metabolism*
  • C-Reactive Protein / pharmacology
  • Cells, Cultured
  • Epithelial Cells / drug effects
  • Epithelial Cells / metabolism
  • Female
  • Humans
  • Leukocytes, Mononuclear / metabolism
  • Lipopolysaccharides / pharmacology
  • Macrophages, Alveolar / metabolism
  • Male
  • Matrix Metalloproteinase 9 / biosynthesis*
  • Pulmonary Disease, Chronic Obstructive / metabolism*
  • Pulmonary Disease, Chronic Obstructive / surgery
  • Smoking / metabolism
  • Young Adult


  • Antibodies, Blocking
  • Lipopolysaccharides
  • Basigin
  • C-Reactive Protein
  • Matrix Metalloproteinase 9