Removing the Vitelline Membrane from Xenopus laevis Embryos

Hazel L Sive; Robert M Grainger; Richard M Harland

doi:10.1101/pdb.prot4732

Removing the Vitelline Membrane from Xenopus laevis Embryos

CSH Protoc. 2007 May 1:2007:pdb.prot4732. doi: 10.1101/pdb.prot4732.

Authors

Hazel L Sive, Robert M Grainger, Richard M Harland

PMID: 21357077
DOI: 10.1101/pdb.prot4732

Abstract

INTRODUCTIONThis protocol presents a method for the removal of the vitelline membrane from Xenopus embryos, which is essential for embryo dissection experiments. Removal of the vitelline membrane is a technique that becomes easier with practice. The easiest embryos on which to learn this technique are those with a large space between the membrane and the embryo, that is, late neurula stages.