A primer to scaffolded DNA origami

Nat Methods. 2011 Mar;8(3):221-9. doi: 10.1038/nmeth.1570.


Molecular self-assembly with scaffolded DNA origami enables building custom-shaped nanometer-scale objects with molecular weights in the megadalton regime. Here we provide a practical guide for design and assembly of scaffolded DNA origami objects. We also introduce a computational tool for predicting the structure of DNA origami objects and provide information on the conditions under which DNA origami objects can be expected to maintain their structure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Computer-Aided Design*
  • DNA / chemistry*
  • DNA / ultrastructure
  • DNA Primers / chemistry*
  • Nanoparticles / chemistry*
  • Nanoparticles / ultrastructure
  • Nucleic Acid Conformation


  • DNA Primers
  • DNA