Abstract
The fluorescent protein aptly named "Killer Red" (KRed) is capable of killing transfected cells and inactivating fused proteins upon exposure to visible light in the presence of oxygen. We have investigated the source of the bioactive species through a variety of photophysical and photochemical techniques. Our results indicate a Type I (electron transfer mediated) photosensitizing mechanism.
© The Royal Society of Chemistry 2011
Publication types
-
Research Support, N.I.H., Extramural
-
Research Support, Non-U.S. Gov't
-
Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
-
Antioxidants / metabolism
-
Electron Transport
-
Green Fluorescent Proteins / chemistry
-
Green Fluorescent Proteins / metabolism*
-
Humans
-
Light
-
Luminescent Proteins / chemistry
-
Luminescent Proteins / metabolism*
-
Oxidation-Reduction
-
Photochemistry / methods*
-
Photochemotherapy / methods
-
Reactive Oxygen Species / metabolism
Substances
-
Antioxidants
-
Luminescent Proteins
-
Reactive Oxygen Species
-
killer red protein, Anthomedusae
-
red fluorescent protein
-
Green Fluorescent Proteins