Quality control in LC-MS/MS

Proteomics. 2011 Mar;11(6):1026-30. doi: 10.1002/pmic.201000578. Epub 2011 Feb 7.


In the last 15 years, MS-based protein characterization has expanded at a rapid rate. This success is built upon constantly improving instrumentation and a variety of ingenious methods applied to numerous biological questions. However, the reproducibility of mass spectrometric results is considered by many as insufficient. In part, inadequate quality control might be responsible for the lack of reproducibility. Quality control is rarely discussed in scientific publications. Here, we briefly present measures undertaken in our laboratory to foster a general discussion of the subject.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, Liquid / methods
  • Chromatography, Liquid / standards
  • Data Interpretation, Statistical
  • Proteomics / methods
  • Proteomics / standards*
  • Proteomics / statistics & numerical data
  • Quality Control
  • Reproducibility of Results
  • Systems Biology
  • Tandem Mass Spectrometry / methods
  • Tandem Mass Spectrometry / standards*
  • Tandem Mass Spectrometry / statistics & numerical data