Heat shock response in yeast involves changes in both transcription rates and mRNA stabilities

PLoS One. 2011 Feb 25;6(2):e17272. doi: 10.1371/journal.pone.0017272.

Abstract

We have analyzed the heat stress response in the yeast Saccharomyces cerevisiae by determining mRNA levels and transcription rates for the whole transcriptome after a shift from 25 °C to 37 °C. Using an established mathematical algorithm, theoretical mRNA decay rates have also been calculated from the experimental data. We have verified the mathematical predictions for selected genes by determining their mRNA decay rates at different times during heat stress response using the regulatable tetO promoter. This study indicates that the yeast response to heat shock is not only due to changes in transcription rates, but also to changes in the mRNA stabilities. mRNA stability is affected in 62% of the yeast genes and it is particularly important in shaping the mRNA profile of the genes belonging to the environmental stress response. In most cases, changes in transcription rates and mRNA stabilities are homodirectional for both parameters, although some interesting cases of antagonist behavior are found. The statistical analysis of gene targets and sequence motifs within the clusters of genes with similar behaviors shows that both transcriptional and post-transcriptional regulons apparently contribute to the general heat stress response by means of transcriptional factors and RNA binding proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cluster Analysis
  • Gene Expression Regulation, Fungal
  • Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / metabolism
  • Heat-Shock Response / genetics*
  • Heat-Shock Response / physiology*
  • Models, Biological
  • Organisms, Genetically Modified
  • RNA Stability / genetics
  • RNA Stability / physiology*
  • RNA, Fungal / genetics
  • RNA, Fungal / metabolism
  • RNA, Messenger / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae / physiology
  • Transcription Factors / genetics
  • Transcription Factors / metabolism
  • Transcription Factors / physiology
  • Transcription, Genetic*
  • Yeasts / genetics*
  • Yeasts / metabolism*

Substances

  • Heat-Shock Proteins
  • RNA, Fungal
  • RNA, Messenger
  • Transcription Factors

Associated data

  • GEO/GSE24488