Focusing transgene expression in Drosophila by coupling Gal4 with a novel split-LexA expression system

Genetics. 2011 May;188(1):229-33. doi: 10.1534/genetics.110.126193. Epub 2011 Mar 2.

Abstract

Here we report the development of a ternary version of the LexA::VP16/LexAop system in which the DNA-binding and trans-activating moieties are independently targeted using distinct promoters to achieve highly restricted, intersectional expression patterns. This Split LexA system can be concatenated with the Gal4/upstream activating sequence system to refine the expression patterns of existing Gal4 lines with minimal genetic manipulations.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Bacterial Proteins / metabolism*
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / genetics*
  • Gene Expression*
  • Promoter Regions, Genetic / genetics
  • Serine Endopeptidases / metabolism*
  • Transcription Factors / metabolism*
  • Transgenes / genetics*

Substances

  • Bacterial Proteins
  • Drosophila Proteins
  • GAL4 protein, Drosophila
  • LexA protein, Bacteria
  • Transcription Factors
  • Serine Endopeptidases