Abstract
Here we report the development of a ternary version of the LexA::VP16/LexAop system in which the DNA-binding and trans-activating moieties are independently targeted using distinct promoters to achieve highly restricted, intersectional expression patterns. This Split LexA system can be concatenated with the Gal4/upstream activating sequence system to refine the expression patterns of existing Gal4 lines with minimal genetic manipulations.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, N.I.H., Intramural
MeSH terms
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Animals
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Bacterial Proteins / metabolism*
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Drosophila Proteins / metabolism*
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Drosophila melanogaster / genetics*
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Gene Expression*
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Promoter Regions, Genetic / genetics
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Serine Endopeptidases / metabolism*
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Transcription Factors / metabolism*
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Transgenes / genetics*
Substances
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Bacterial Proteins
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Drosophila Proteins
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GAL4 protein, Drosophila
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LexA protein, Bacteria
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Transcription Factors
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Serine Endopeptidases