Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1990 Jan;9(1):251-5.
doi: 10.1002/j.1460-2075.1990.tb08102.x.

Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes

A Bindereif  1 , T Wolff, M R Green
Affiliations

Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes

A Bindereif et al. EMBO J. 1990 Jan.

Abstract

U6 snRNA sequences required for assembly of U4/U6 snRNP and splicing complexes were determined by in vitro reconstitution of snRNPs. Both mutagenesis and chemical modification/interference assays identify a U6 snRNA domain required for U4/U6 snRNP formation. The results support the existence of a U4/U6 snRNA interaction domain previously proposed on the basis of phylogenetic evidence. In addition, two short U6 snRNA regions flanking the U4/U6 interaction domain are essential to assemble the U4/U6 snRNP into splicing complexes. These two regions may represent binding sites for splicing factors or may facilitate the formation of an alternative U6 snRNA secondary structure during spliceosome assembly.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Nature. 1986 Nov 27-Dec 3;324(6095):341-5 - PubMed
    1. Cell. 1987 Jun 19;49(6):763-74 - PubMed
    1. EMBO J. 1987 Aug;6(8):2415-24 - PubMed
    1. Genes Dev. 1987 Nov;1(9):1014-27 - PubMed
    1. Proc Natl Acad Sci U S A. 1988 Jan;85(2):411-5 - PubMed

Publication types

Substances