Encapsulated enhanced green fluorescence protein in silica nanoparticle for cellular imaging

Zhengwei Cai; Zhangmei Ye; Xiaowei Yang; Yanli Chang; Haifang Wang; Yuanfang Liu; Aoneng Cao

doi:10.1039/c0nr00956c

Encapsulated enhanced green fluorescence protein in silica nanoparticle for cellular imaging

Nanoscale. 2011 May;3(5):1974-6. doi: 10.1039/c0nr00956c. Epub 2011 Mar 3.

Authors

Zhengwei Cai¹, Zhangmei Ye, Xiaowei Yang, Yanli Chang, Haifang Wang, Yuanfang Liu, Aoneng Cao

Affiliation

¹ Institute of Nanochemistry and Nanobiology, Shanghai University, Shanghai, 200444, China.

PMID: 21369623
DOI: 10.1039/c0nr00956c

Abstract

The enhanced green fluorescence protein (EGFP) is efficiently encapsulated in silica nanoparticle by multiple covalent bonds. The silica encapsulation greatly increases EGFP's fluorescence intensity and stabilities against protease, denaturant and heat, making it a potential fluorescence probe for cellular imaging.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cells, Cultured / cytology*
Drug Carriers / chemistry*
Green Fluorescent Proteins / analysis*
Green Fluorescent Proteins / chemistry*
Microscopy, Fluorescence / methods*
Nanocapsules / chemistry*
Silicon Dioxide / chemistry*

Substances

Drug Carriers
Nanocapsules
Green Fluorescent Proteins
Silicon Dioxide