Surfaces of Murine Lymphocyte Subsets Differ in Sialylation States and Antigen Distribution of a Major N-linked Penultimate Saccharide Structure

Cell Immunol. 1990 Feb;125(2):337-53. doi: 10.1016/0008-8749(90)90089-a.

Abstract

Rat liver beta-galactoside alpha-2,6-sialyltransferase and Vibrio cholerae sialidase were used with cytidine-5'-monophospho-N-acetyl-[3H]neuraminic acid (CMP-[3H]NeuAc) to specifically probe the distribution and sialylation state of Gal beta 1-4GlcNAc residues on N-linked saccharides on the surfaces of murine lymphocytes. The relative extent of exogenous sialyltransferase-mediated sialylation (per cellular protein) was thymocytes greater than T-cells greater than T-cell lymphoma (EL-4) greater than B-cells greater than B-cell lymphoma (AKTB-1b) greater than splenocytes. Prior desialylation increased exogenous resialylation by 23.8-, 13.1-, 7.1-, 7.9-, 7.0-, and 5.3-fold for splenocytes, B-cells, T-cells, EL-4, AKTB-1b, and thymocytes, respectively. Though numerous glycoproteins were labeled, the majority of the Gal beta 1-4GlcNAc residues were detected on a relatively small number of cell surface proteins, many of which are well-defined lymphocyte antigens. Gal beta 1-4GlcNAc residues on thymocytes were found to exist in an undersialylated state on T200 but not on other antigens (e.g., Thy-1). T200 was found to be fully sialylated on mature cells (i.e., hydrocortisone-resistant thymocytes and splenic T-cells), suggesting that its sialylation state is developmentally regulated. These studies indicate that the number, sialylation state, and polypeptide distribution of the penultimate structure, Gal beta 1-4GlcNAc, differ on N-linked saccharides on the surfaces of different lymphocyte populations.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Differentiation / analysis
  • Antigens, Surface / analysis*
  • Electrophoresis, Polyacrylamide Gel
  • Glycoconjugates / analysis
  • Histocompatibility Antigens / analysis
  • Leukocyte Common Antigens
  • Lymphocytes / analysis*
  • Lymphocytes / immunology
  • Mice
  • Mice, Inbred C3H
  • N-Acetylneuraminic Acid
  • Oligosaccharides / metabolism*
  • Sialic Acids / metabolism*
  • Sialyltransferases / pharmacology

Substances

  • Antigens, Differentiation
  • Antigens, Surface
  • Glycoconjugates
  • Histocompatibility Antigens
  • Oligosaccharides
  • Sialic Acids
  • Sialyltransferases
  • Leukocyte Common Antigens
  • N-Acetylneuraminic Acid