Abstract
The cloning and characterization of a cDNA encoding a calreticulin from the pinewood nematode Bursaphelenchus xylophilus is described herein. The full-length cDNA (Bx-crt-1) contained a 1200 bp open reading frame that could be translated to a 399 amino acid polypeptide. The deduced protein contained highly conserved regions of a calreticulin gene and had 66.2-70.1% amino acid sequence identity to other calreticulin sequences from nematodes. RNAi, RT-PCR amplification, and southern blot suggest that Bx-crt-1 may be important for the development of B. xylophilus.
Copyright © 2011 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Blotting, Southern
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Calreticulin / chemistry
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Calreticulin / genetics*
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Calreticulin / metabolism
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Cloning, Molecular
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DNA Primers
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DNA, Complementary / chemistry
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DNA, Complementary / genetics*
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DNA, Helminth / chemistry
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DNA, Helminth / genetics
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Female
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Gene Expression Regulation, Developmental
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Molecular Sequence Data
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Pinus / parasitology*
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Plant Diseases / parasitology*
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RNA Interference
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RNA, Helminth / isolation & purification
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Sequence Alignment
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Sequence Analysis, DNA
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Tylenchida / chemistry
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Tylenchida / genetics*
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Tylenchida / pathogenicity
Substances
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Calreticulin
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DNA Primers
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DNA, Complementary
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DNA, Helminth
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RNA, Helminth