Abstract
A monoclonal antibody raised against mammalian spliceosomes specifically recognizes a non-snRNP factor required for spliceosome assembly. This splicing factor is highly concentrated in discrete regions within the nucleus, in a pattern that is a distinct subset of that seen with anti-snRNP antibodies. These observations are evidence that spliceosome assembly could be compartmentalized within the nucleus.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Monoclonal
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Antigens / analysis
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Antigens / immunology
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Cell Nucleus / analysis
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Cell Nucleus / ultrastructure*
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Fluorescent Antibody Technique
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Immunosorbent Techniques
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Microscopy, Electron
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Nuclear Proteins / analysis*
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Nuclear Proteins / immunology
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Nuclear Proteins / physiology
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RNA Precursors / genetics
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RNA Splicing*
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RNA, Messenger / genetics
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Ribonucleoproteins / analysis
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Ribonucleoproteins, Small Nuclear
Substances
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Antibodies, Monoclonal
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Antigens
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Nuclear Proteins
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RNA Precursors
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RNA, Messenger
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Ribonucleoproteins
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Ribonucleoproteins, Small Nuclear