Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus

Nature. 1990 Feb 1;343(6257):437-41. doi: 10.1038/343437a0.

Abstract

A monoclonal antibody raised against mammalian spliceosomes specifically recognizes a non-snRNP factor required for spliceosome assembly. This splicing factor is highly concentrated in discrete regions within the nucleus, in a pattern that is a distinct subset of that seen with anti-snRNP antibodies. These observations are evidence that spliceosome assembly could be compartmentalized within the nucleus.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antigens / analysis
  • Antigens / immunology
  • Cell Nucleus / analysis
  • Cell Nucleus / ultrastructure*
  • Fluorescent Antibody Technique
  • Immunosorbent Techniques
  • Microscopy, Electron
  • Nuclear Proteins / analysis*
  • Nuclear Proteins / immunology
  • Nuclear Proteins / physiology
  • RNA Precursors / genetics
  • RNA Splicing*
  • RNA, Messenger / genetics
  • Ribonucleoproteins / analysis
  • Ribonucleoproteins, Small Nuclear

Substances

  • Antibodies, Monoclonal
  • Antigens
  • Nuclear Proteins
  • RNA Precursors
  • RNA, Messenger
  • Ribonucleoproteins
  • Ribonucleoproteins, Small Nuclear