Abstract
Bioactivity-guided isolation on a n-hexane-soluble fraction of Thyrsanthera suborbicularis led to the isolation of a new rosane-type diterpene, 19-hydroxy-1(10), 15-rosadiene (1), along with three known compounds, taraxerol, acetyl aleuritolic acid, and spathulenol. The structures of isolated compounds were determined by interpretation of NMR spectroscopic data and mass spectrometry. Compound 1 demonstrated significantly inhibitory activity on nitric oxide production in RAW264.7 lipopolysaccharide (LPS)-activated mouse macrophages with an IC(50) value of 2.91 µg/ml via the suppression of inducible nitric oxide synthase (iNOS) mRNA expression.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents / chemistry*
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Anti-Inflammatory Agents / isolation & purification
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Cell Line, Tumor
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Diterpenes / chemistry*
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Diterpenes / isolation & purification
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Euphorbiaceae / chemistry*
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Lipopolysaccharides / toxicity
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Macrophages / drug effects
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Macrophages / metabolism
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Magnetic Resonance Spectroscopy
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Mass Spectrometry
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Mice
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Molecular Conformation
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Nitric Oxide / metabolism
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Nitric Oxide Synthase Type II / genetics
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Nitric Oxide Synthase Type II / metabolism
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RNA, Messenger / metabolism
Substances
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Anti-Inflammatory Agents
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Diterpenes
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Lipopolysaccharides
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RNA, Messenger
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Nitric Oxide
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Nitric Oxide Synthase Type II