Dynamic clamp with StdpC software

Nat Protoc. 2011 Mar;6(3):405-17. doi: 10.1038/nprot.2010.200. Epub 2011 Mar 3.


Dynamic clamp is a powerful method that allows the introduction of artificial electrical components into target cells to simulate ionic conductances and synaptic inputs. This method is based on a fast cycle of measuring the membrane potential of a cell, calculating the current of a desired simulated component using an appropriate model and injecting this current into the cell. Here we present a dynamic clamp protocol using free, fully integrated, open-source software (StdpC, for spike timing-dependent plasticity clamp). Use of this protocol does not require specialist hardware, costly commercial software, experience in real-time operating systems or a strong programming background. The software enables the configuration and operation of a wide range of complex and fully automated dynamic clamp experiments through an intuitive and powerful interface with a minimal initial lead time of a few hours. After initial configuration, experimental results can be generated within minutes of establishing cell recording.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Computer Systems
  • Electric Stimulation
  • Electrophysiology / methods*
  • Membrane Potentials
  • Microelectrodes
  • Models, Neurological
  • Neurons / physiology
  • Patch-Clamp Techniques / instrumentation
  • Patch-Clamp Techniques / methods*
  • Software*
  • Synapses / physiology