Nanoscale spectroscopy and imaging of hemoglobin

J Biophotonics. 2011 Sep;4(9):588-91. doi: 10.1002/jbio.201100003. Epub 2011 Mar 4.

Abstract

Sub diffraction limited infrared absorption imaging of hemoglobin was performed by coupling IR optics with an atomic force microscope. Comparisons between the AFM topography and IR absorption images of micron sized hemoglobin features are presented, along with nanoscale IR spectroscopic analysis of the metalloprotein.

Publication types

  • Evaluation Study
  • Letter
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Absorption
  • Hemoglobins / chemistry*
  • Hemoglobins / ultrastructure
  • Humans
  • Image Processing, Computer-Assisted / instrumentation
  • Image Processing, Computer-Assisted / methods*
  • Lasers
  • Metalloproteins / chemistry
  • Metalloproteins / ultrastructure
  • Microscopy, Atomic Force / instrumentation
  • Microscopy, Atomic Force / methods*
  • Nanostructures / chemistry*
  • Nanostructures / ultrastructure
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectroscopy, Fourier Transform Infrared / instrumentation
  • Spectroscopy, Fourier Transform Infrared / methods*

Substances

  • Hemoglobins
  • Metalloproteins