A simple tool for stereological assessment of digital images: the STEPanizer

J Microsc. 2011 Jul;243(1):47-59. doi: 10.1111/j.1365-2818.2010.03481.x. Epub 2011 Mar 7.


STEPanizer is an easy-to-use computer-based software tool for the stereological assessment of digitally captured images from all kinds of microscopical (LM, TEM, LSM) and macroscopical (radiology, tomography) imaging modalities. The program design focuses on providing the user a defined workflow adapted to most basic stereological tasks. The software is compact, that is user friendly without being bulky. STEPanizer comprises the creation of test systems, the appropriate display of digital images with superimposed test systems, a scaling facility, a counting module and an export function for the transfer of results to spreadsheet programs. Here we describe the major workflow of the tool illustrating the application on two examples from transmission electron microscopy and light microscopy, respectively.

MeSH terms

  • Animals
  • Image Processing, Computer-Assisted / methods*
  • Liver / pathology
  • Lung / pathology
  • Microscopy / methods*
  • Rats