The protective effect of sodium nitroprusside (SNP) treatment on Arabidopsis thaliana seedlings exposed to toxic level of Pb is not linked to avoidance of Pb uptake

Ecotoxicol Environ Saf. 2011 Jul;74(5):1310-5. doi: 10.1016/j.ecoenv.2011.02.006. Epub 2011 Mar 3.

Abstract

The effects of 100 μM Pb(NO₃)₂ on 7-day-old Arabidopsis thaliana seedlings grown from seeds pretreated with a nitric oxide donor, sodium nitroprusside (SNP), or a nitric oxide scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (cPTIO) were investigated. Physiological stress induced by Pb (reduced root growth) was less evident in seedlings grown from seeds pretreated with SNP. However, SNP pretreatment of seeds did not affect Pb accumulation in the seedlings. Pb exposure caused oxidative stress by elevating hydrogen peroxide (H₂O₂) and lipid hydroperoxide contents of the seedlings. SNP pretreatment of seeds counteracted Pb toxicity by reducing the H₂O₂ and lipid hydroperoxide contents of Pb-exposed seedlings. Additionally, Pb-induced rises in antioxidative enzyme activities were reversed by SNP pretreatment of seeds. It was concluded that the ameliorating effects of SNP pretreatment were associated with the release of nitric oxide because cPTIO reversed these effects of SNP pretreatment of seeds and more importantly SNP pretreatment did not trigger an avoidance mechanism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antioxidants / pharmacology*
  • Arabidopsis / drug effects*
  • Arabidopsis / metabolism
  • Benzoates / pharmacology
  • Biodegradation, Environmental
  • Catalase / metabolism
  • Glutathione Peroxidase / metabolism
  • Hydrogen Peroxide / metabolism
  • Imidazoles / pharmacology
  • Lead / metabolism
  • Lead / toxicity*
  • Nitric Oxide / metabolism
  • Nitric Oxide Donors / pharmacology
  • Nitroprusside / pharmacology*
  • Oxidative Stress / drug effects
  • Plant Roots / drug effects
  • Plant Roots / metabolism
  • Reactive Oxygen Species / metabolism
  • Seedlings / drug effects*
  • Seedlings / metabolism
  • Seeds / drug effects
  • Seeds / metabolism
  • Soil Pollutants / metabolism
  • Soil Pollutants / toxicity*
  • Superoxide Dismutase / metabolism

Substances

  • Antioxidants
  • Benzoates
  • Imidazoles
  • Nitric Oxide Donors
  • Reactive Oxygen Species
  • Soil Pollutants
  • 1,3-dihydroxy-4,4,5,5-tetramethyl-2-(4-carboxyphenyl)tetrahydroimidazole
  • Nitroprusside
  • Lead
  • Nitric Oxide
  • Hydrogen Peroxide
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase