Exocytosis at the hair cell ribbon synapse apparently operates without neuronal SNARE proteins

Nat Neurosci. 2011 Apr;14(4):411-3. doi: 10.1038/nn.2774. Epub 2011 Mar 6.

Abstract

SNARE proteins mediate membrane fusion. Neurosecretion depends on neuronal soluble NSF attachment protein receptors (SNAREs; SNAP-25, syntaxin-1, and synaptobrevin-1 or synaptobrevin-2) and is blocked by neurotoxin-mediated cleavage or genetic ablation. We found that exocytosis in mouse inner hair cells (IHCs) was insensitive to neurotoxins and genetic ablation of neuronal SNAREs. mRNA, but no synaptically localized protein, of neuronal SNAREs was present in IHCs. Thus, IHC exocytosis is unconventional and may operate independently of neuronal SNAREs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • Cells, Cultured
  • Exocytosis / drug effects
  • Exocytosis / physiology*
  • Hair Cells, Auditory, Inner / drug effects
  • Hair Cells, Auditory, Inner / metabolism*
  • Hair Cells, Auditory, Inner / ultrastructure
  • Mice
  • Mice, Knockout
  • Mice, Mutant Strains
  • Mice, Transgenic
  • Organ Culture Techniques
  • SNARE Proteins / deficiency
  • SNARE Proteins / genetics*
  • Synapses / drug effects
  • Synapses / metabolism*
  • Synapses / ultrastructure

Substances

  • SNARE Proteins