Simultaneous Determination of N-benzylpiperazine and 1-(3-trifluoromethylphenyl)piperazine in Rat Plasma by HPLC-fluorescence Detection and Its Application to Monitoring of These Drugs

Biomed Chromatogr. 2012 Jan;26(1):21-5. doi: 10.1002/bmc.1619. Epub 2011 Mar 9.


An HPLC-fluorescence detection method for simultaneous determination of N-benzylpiperazine (BZP) and 1-(3-trifluoromethylphenyl)piperazine (TFMPP) labeled with 4-(4,5-diphenyl-1 H-imidazol-2-yl)benzoyl chloride (DIB-Cl) was described. DIB-BZP and -TFMPP were well separated within 13 min without interference of peaks from plasma components. The lower detection limits of BZP and TFMPP at a signal-to-noise ratio of 3 were 0.9 and 4.6 ng/mL, respectively. Precisions of the proposed method for intra- and inter-day assays were less than 4.8 and 9.1% as %RSD (n =5). Furthermore, the method could be successfully applied to monitor both compounds in plasma after their sole or co-administration to rats (each dose, 2 mg/kg). Clearance of TFMPP was significantly different under the conditions (P=0.047).

MeSH terms

  • Animals
  • Benzoates
  • Chromatography, High Pressure Liquid / methods*
  • Drug Monitoring
  • Imidazoles
  • Limit of Detection
  • Male
  • Piperazines / blood*
  • Piperazines / pharmacokinetics
  • Rats
  • Rats, Wistar
  • Reproducibility of Results
  • Spectrometry, Fluorescence


  • 4-(4,5-diphenyl-1H-imidazol-2-yl)benzoyl chloride
  • Benzoates
  • Imidazoles
  • N-benzylpiperazine
  • Piperazines
  • 1-(3-trifluoromethylphenyl)piperazine