A comprehensive investigation of anti-inflammatory diarylheptanoids from the leaves of Alnus formosana

Phytochemistry. 2012 Jan;73(1):84-94. doi: 10.1016/j.phytochem.2011.02.008. Epub 2011 Mar 7.

Abstract

This study was aimed to investigate thoroughly the diarylheptanoids in the n-BuOH soluble fraction of leaves of Alnus formosana in order to examine their anti-inflammatory activities. The application of HPLC-SPE-NMR as a preliminary chemical screening led to characterization of eleven compounds. Further separation resulted in isolation of 28 compounds, of which 10 diarylheptanoids and 2-coumaroylxyloside are new natural products. Compound 1 and alnuside A (27) were found to possess good activities against LPS-induced NO production with respective IC(50) values of 7.99 and 8.08 μM, and which were devoid of significant cytotoxicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alnus / chemistry*
  • Animals
  • Anti-Inflammatory Agents / chemistry
  • Anti-Inflammatory Agents / isolation & purification*
  • Anti-Inflammatory Agents / pharmacology*
  • Chromatography, High Pressure Liquid
  • Diarylheptanoids / chemistry
  • Diarylheptanoids / isolation & purification*
  • Diarylheptanoids / pharmacology*
  • Inhibitory Concentration 50
  • Lipopolysaccharides / pharmacology
  • Mice
  • Molecular Structure
  • Nitric Oxide / biosynthesis
  • Nuclear Magnetic Resonance, Biomolecular
  • Plant Leaves / chemistry
  • Taiwan

Substances

  • Anti-Inflammatory Agents
  • Diarylheptanoids
  • Lipopolysaccharides
  • Nitric Oxide