Isolation and identification of human brain natriuretic peptides in cardiac atrium

Biochem Biophys Res Commun. 1990 Mar 16;167(2):693-700. doi: 10.1016/0006-291x(90)92081-a.


By using a radioimmunoassay (RIA) system newly established for human brain natriuretic peptide (BNP), a high concentration of immunoreactive (ir-) human BNP (hBNP) has been found in cardiac atrium (1). Two molecular forms of ir-hBNP of 4K and 13-15K were isolated from atrial extracts by using anti-hBNP IgG immunoaffinity chromatography and reverse phase high performance liquid chromatography (HPLC). By microsequencing, the peptides were determined to be a pro-hBNP (gamma-hBNP) and its C-terminal 32-amino acid peptide (hBNP-32). Based on these results, in cardiac atrium, hBNP is found to be processed in a pathway similar to that of porcine BNP (pBNP) but distinct from that of rat BNP, although low MW hBNP-32 is a major form in contrast to pBNP which exists as a high MW gamma-pBNP.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / analysis
  • Animals
  • Atrial Natriuretic Factor / analysis
  • Chromatography, Affinity
  • Chromatography, Gel
  • Heart Atria / analysis
  • Humans
  • Molecular Sequence Data
  • Myocardium / analysis*
  • Natriuretic Peptide, Brain
  • Nerve Tissue Proteins / analysis
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / isolation & purification*
  • Radioimmunoassay
  • Rats
  • Swine


  • Amino Acids
  • Nerve Tissue Proteins
  • Natriuretic Peptide, Brain
  • Atrial Natriuretic Factor