Higher hypochlorous acid scavenging activity of ethyl pyruvate compared to its sodium salt

Biosci Biotechnol Biochem. 2011;75(3):500-4. doi: 10.1271/bbb.100728. Epub 2011 Mar 7.


Although a number of studies have focused on the higher ethyl pyruvate antioxidative activity than its sodium salt under various stress conditions, and the greater protective properties of the ester form have been suggested as the effect of better cell membrane penetration, the molecular mechanism has remained unclear. The aim of the present study was therefore to compare the antioxidative activities of sodium and ethyl pyruvate under in vitro conditions by using a liver homogenate as the model for cell membrane transport deletion. The potential effect of ethanol was also evaluated, and hypochlorous acid was used as an oxidant. Our data indicate the concentration-dependent scavenging potency of both sodium and ethyl pyruvate, with the ester having higher activity. This effect was not related to the presence of ethanol. Better protection of the liver homogenate by ethyl pyruvate was also apparent, despite the fact that cell membrane transport was omitted.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzofurans / analysis
  • Benzothiazoles / analysis
  • Biological Transport
  • Cell-Free System / drug effects*
  • Cell-Free System / metabolism
  • Esters / pharmacology*
  • Ethanol / metabolism
  • Hypochlorous Acid / adverse effects
  • Liver / drug effects
  • Liver / metabolism
  • Models, Biological
  • Oxidation-Reduction / drug effects*
  • Oxidative Stress / drug effects
  • Permeability
  • Pyruvates / pharmacology*
  • Rats
  • Rats, Wistar
  • Sodium / metabolism
  • Sulfonic Acids / analysis


  • Benzofurans
  • Benzothiazoles
  • Esters
  • Pyruvates
  • Sulfonic Acids
  • ethyl pyruvate
  • 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
  • Ethanol
  • 1,3-diphenylisobenzofuran
  • Hypochlorous Acid
  • Sodium