Hydrogen-rich saline protects against renal ischemia/reperfusion injury in rats

J Surg Res. 2011 May 15;167(2):e339-44. doi: 10.1016/j.jss.2010.11.005. Epub 2010 Dec 2.


Background: Recently it has been demonstrated that hydrogen, as a novel antioxidant, can selectively reduce hydroxyl radicals (·OH) and peroxynitrite anion (ONOO-) in vitro and exert therapeutic antioxidant activity in many diseases. This study was designed to investigate the effect of hydrogen-rich saline on renal ischemia/reperfusion (I/R) injury in rats.

Methods: A rat model of renal I/R injury was induced by 45-min occlusion of the bilateral renal pedicles and 24-h reperfusion. Physiologic saline, hydrogen-rich saline, or nitrogen-rich saline (8 mL/kg) were administered intraperitoneally at 5 min before reperfusion, respectively.

Results: After I/R injury, serum blood urea nitrogen (BUN), creatinine (Cr), tissue malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OhdG), TNF-α, IL-1β, IL-6 levels, and myeloperoxidase (MPO) activity were all increased significantly, while tissue superoxide dismutase (SOD) and catalase (CAT) activities were all decreased significantly. Hydrogen-rich saline reversed these changes and relieved morphological renal injury and I/R-induced apoptosis, while no significant changes were observed in the nitrogen-rich saline-treated group compared with physiologic saline-treated group.

Conclusions: Hydrogen-rich saline is able to attenuate the renal I/R injury, which is possibly by reduction of oxidative stress and inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Animals
  • Cytokines / metabolism
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / metabolism
  • Disease Models, Animal
  • Hydrogen / therapeutic use*
  • Kidney / blood supply*
  • Kidney / metabolism
  • Kidney / physiopathology*
  • Male
  • Malondialdehyde / metabolism
  • Neutrophil Infiltration / drug effects
  • Neutrophil Infiltration / physiology
  • Oxidative Stress / drug effects
  • Oxidative Stress / physiology
  • Rats
  • Rats, Sprague-Dawley
  • Reperfusion Injury / physiopathology
  • Reperfusion Injury / prevention & control*
  • Sodium Chloride / therapeutic use*


  • Cytokines
  • Sodium Chloride
  • Malondialdehyde
  • Hydrogen
  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine