A midzone-based ruler adjusts chromosome compaction to anaphase spindle length

Science. 2011 Apr 22;332(6028):465-8. doi: 10.1126/science.1201578. Epub 2011 Mar 10.

Abstract

Partitioning of chromatids during mitosis requires that chromosome compaction and spindle length scale appropriately with each other. However, it is not clear whether chromosome condensation and spindle elongation are linked. Here, we find that yeast cells could cope with a 45% increase in the length of their longest chromosome arm by increasing its condensation. The spindle midzone, aurora/Ipl1 activity, and Ser10 of histone H3 mediated this response. Thus, the anaphase spindle may function as a ruler to adapt the condensation of chromatids, promoting their segregation regardless of chromosome or spindle length.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aldose-Ketose Isomerases / genetics
  • Anaphase*
  • Aurora Kinases
  • Chromosome Segregation
  • Chromosomes, Fungal / genetics
  • Chromosomes, Fungal / physiology*
  • Histones / metabolism
  • Intracellular Signaling Peptides and Proteins / genetics
  • Intracellular Signaling Peptides and Proteins / metabolism
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism
  • Mutation
  • Protein Serine-Threonine Kinases / genetics
  • Protein Serine-Threonine Kinases / metabolism
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / physiology*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Spindle Apparatus / physiology*
  • Spindle Apparatus / ultrastructure*

Substances

  • Histones
  • Intracellular Signaling Peptides and Proteins
  • Microtubule-Associated Proteins
  • Saccharomyces cerevisiae Proteins
  • Sli15 protein, S cerevisiae
  • Slk19 protein, S cerevisiae
  • Aurora Kinases
  • IPL1 protein, S cerevisiae
  • Protein Serine-Threonine Kinases
  • Aldose-Ketose Isomerases
  • TRP1 protein, S cerevisiae