Rapid and efficient detection of Mycobacterium tuberculosis in respiratory and non-respiratory samples

Int J Tuberc Lung Dis. 2011 Apr;15(4):553-5. doi: 10.5588/ijtld.10.0497.


The detection of Mycobacterium tuberculosis using a new commercial real-time polymerase chain reaction (PCR) assay (Xpert™ MTB/RIF) was evaluated on 91 respiratory and 89 non-respiratory samples recovered from 132 patients suspected of tuberculosis (TB). Overall, 31 (17.2%) of the 180 samples, including 17 respiratory and 14 non-respiratory (respectively 17 and 12 PCR-positive), yielded M. tuberculosis on culture. The sensitivity and specificity of PCR were respectively 100% and 100%, and 85.7% and 97.3% for respiratory and non-respiratory samples. Although the test is validated only for respiratory samples, our findings suggest that it could be useful for the diagnosis of extra-pulmonary TB.

Publication types

  • Validation Study

MeSH terms

  • Female
  • Humans
  • Male
  • Middle Aged
  • Mycobacterium tuberculosis / isolation & purification*
  • Polymerase Chain Reaction / methods*
  • Prospective Studies
  • Sensitivity and Specificity
  • Tuberculosis / diagnosis*
  • Tuberculosis / microbiology