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. 1990 May;172(5):2782-4.
doi: 10.1128/jb.172.5.2782-2784.1990.

An Upstream uncD Sequence Modulates Translation of Escherichia Coli uncC

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Free PMC article

An Upstream uncD Sequence Modulates Translation of Escherichia Coli uncC

S D Dunn et al. J Bacteriol. .
Free PMC article

Abstract

The effect of upstream uncD sequences on expression of the Escherichia coli uncC gene, encoding the epsilon subunit of F1-ATPase, was studied. uncC expression was reduced severalfold in plasmid constructs bearing, in addition to uncC, a region of uncD located between 85 and 119 bases upstream from the uncC initiation codon. This reduction was independent of in-frame translation of the uncD sequences. An mRNA stem-loop structure in which sequences located within the inhibitory region of uncD base pair with the uncDC intercistronic region is suggested to function in modulating uncC expression.

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