The AP2/ERF transcription factor WIND1 controls cell dedifferentiation in Arabidopsis

Curr Biol. 2011 Mar 22;21(6):508-14. doi: 10.1016/j.cub.2011.02.020.


Many multicellular organisms have remarkable capability to regenerate new organs after wounding. As a first step of organ regeneration, adult somatic cells often dedifferentiate to reacquire cell proliferation potential, but mechanisms underlying this process remain unknown in plants. Here we show that an AP2/ERF transcription factor, WOUND INDUCED DEDIFFERENTIATION 1 (WIND1), is involved in the control of cell dedifferentiation in Arabidopsis. WIND1 is rapidly induced at the wound site, and it promotes cell dedifferentiation and subsequent cell proliferation to form a mass of pluripotent cells termed callus. We further demonstrate that ectopic overexpression of WIND1 is sufficient to establish and maintain the dedifferentiated status of somatic cells without exogenous auxin and cytokinin, two plant hormones that are normally required for cell dedifferentiation. In vivo imaging of a synthetic cytokinin reporter reveals that wounding upregulates the B-type ARABIDOPSIS RESPONSE REGULATOR (ARR)-mediated cytokinin response and that WIND1 acts via the ARR-dependent signaling pathway to promote cell dedifferentiation. This study provides novel molecular insights into how plants control cell dedifferentiation in response to wounding.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arabidopsis / physiology*
  • Arabidopsis Proteins / metabolism*
  • Cell Dedifferentiation / physiology*
  • Cell Proliferation
  • Genetic Vectors / genetics
  • Homeodomain Proteins / metabolism*
  • Microarray Analysis
  • Microscopy
  • Nuclear Proteins / metabolism*
  • Transcription Factors / metabolism*


  • APETALA2 protein, Arabidopsis
  • Arabidopsis Proteins
  • Homeodomain Proteins
  • Nuclear Proteins
  • Transcription Factors
  • WIND1 protein, Arabidopsis

Associated data

  • GEO/GSE21631