Cypermethrin exposure during puberty induces oxidative stress and endocrine disruption in male mice

Chemosphere. 2011 Jun;84(1):124-30. doi: 10.1016/j.chemosphere.2011.02.034. Epub 2011 Mar 11.

Abstract

Cypermethrin (CYP) is one of the most common contaminants in the ecosystem. The effects of CYP exposure on the induction of oxidative stress and endocrine disruption were studied in adolescent male ICR mice. The hepatic activities of antioxidant enzymes including superoxide dismutase (SOD), glutathione peroxidase (GPX), catalase (CAT) and total antioxidant capacity (T-AOC) increased significantly after 3 weeks (postnatal day 21-42) of oral administration of 20 mg kg(-1) CYP. In accordance with the enzyme activities, the mRNA levels for the genes encoding these antioxidant proteins, such as Sod1, Sod2, Gpx1 and Gpx2, were also up-regulated significantly in the 10 and 20 mg kg(-1) CYP treatment groups. Furthermore, we also found that the 3-week oral administration of CYP decreased transcription levels of key genes in pathways of cholesterol synthesis and transport and testosterone synthesis including HMG-CoA synthase, steroidogenic acute regulatory protein (StAR) and cytochrome P450 17α-hydroxysteroid dehydrogenase (P450 17α in the liver and testes. Serum testosterone levels also decreased significantly in mice after treatment with 20 mg kg(-1) CYP. Taken together, the results indicated that CYP can induce endocrine disruption in adolescent mice. The findings will be helpful in elucidating the mechanism of toxicity induced by CYP in adolescent mice.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Catalase / genetics
  • Catalase / metabolism
  • Cytochrome P-450 Enzyme System / metabolism
  • Endocrine Disruptors / toxicity*
  • Endocrine System / drug effects*
  • Endocrine System / metabolism
  • Gene Expression / drug effects
  • Glutathione Peroxidase / genetics
  • Glutathione Peroxidase / metabolism
  • Hydroxymethylglutaryl-CoA Synthase / genetics
  • Hydroxymethylglutaryl-CoA Synthase / metabolism
  • Hydroxysteroid Dehydrogenases / genetics
  • Hydroxysteroid Dehydrogenases / metabolism
  • Insecticides / toxicity*
  • Liver / drug effects
  • Liver / metabolism
  • Male
  • Mice
  • Mice, Inbred ICR
  • Oxidative Stress
  • Phosphoproteins / genetics
  • Phosphoproteins / metabolism
  • Pyrethrins / toxicity*
  • RNA, Messenger / metabolism
  • Superoxide Dismutase / genetics
  • Superoxide Dismutase / metabolism
  • Testis / drug effects
  • Testis / metabolism

Substances

  • Endocrine Disruptors
  • Insecticides
  • Phosphoproteins
  • Pyrethrins
  • RNA, Messenger
  • steroidogenic acute regulatory protein
  • cypermethrin
  • Cytochrome P-450 Enzyme System
  • Hydroxysteroid Dehydrogenases
  • 3(17)-hydroxysteroid dehydrogenase
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Hydroxymethylglutaryl-CoA Synthase