Molecular Epidemiology of KPC-2- Producing Klebsiella Pneumoniae Isolates in Brazil: The Predominance of Sequence Type 437

Diagn Microbiol Infect Dis. 2011 Jun;70(2):274-7. doi: 10.1016/j.diagmicrobio.2011.01.006. Epub 2011 Mar 11.


The aim of this study was to investigate the genetic relatedness of 57 KPC-2-producing Klebsiella pneumoniae isolates from 5 states in Brazil, during 2006-2009. Pulse-field gel electrophoresis analysis identified 10 pulsotypes. The pulsotype designated as Kp-RJ (Klebsiella pneumoniae-Rio de Janeiro) was the dominant clone found in the states of Rio de Janeiro and Espírito Santo. Multilocus sequence typing of Kp-RJ assigned it to ST 437. Sequence types ST11, ST16, ST25, ST70, ST101, ST105, ST423, ST442, and ST443 were also identified. These results indicate the dissemination of a successful KPC-producing clone (ST437) in Brazil, which is a single locus variant of ST258.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Brazil / epidemiology
  • Electrophoresis, Gel, Pulsed-Field
  • Genotype
  • Humans
  • Klebsiella Infections / epidemiology*
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / classification*
  • Klebsiella pneumoniae / enzymology*
  • Klebsiella pneumoniae / genetics
  • Klebsiella pneumoniae / isolation & purification
  • Molecular Epidemiology
  • Molecular Typing*
  • Multilocus Sequence Typing
  • beta-Lactamases / biosynthesis*


  • beta-Lactamases
  • beta-lactamase KPC-2, Klebsiella pneumoniae