In-gel detection of L-amino acid oxidases based on the visualisation of hydrogen peroxide production

Jan Erik Rau; Ulrich Fischer

doi:10.1016/j.mimet.2011.03.002

In-gel detection of L-amino acid oxidases based on the visualisation of hydrogen peroxide production

J Microbiol Methods. 2011 Jun;85(3):228-9. doi: 10.1016/j.mimet.2011.03.002. Epub 2011 Mar 22.

Authors

Jan Erik Rau¹, Ulrich Fischer

Affiliation

¹ Zentrum für Umweltforschung und nachhaltige Technologien, Fachbereich Biologie/Chemie, Abteilung Marine Mikrobiologie, Universität Bremen, Leobener Strasse, D-28359 Bremen, Germany. jerau@uni-bremen.de

PMID: 21397638
DOI: 10.1016/j.mimet.2011.03.002

Abstract

A simple and practicable method for in-gel detection of bacterial produced L-amino acid oxidases (L-AAOs) after non-denaturing SDS-PAGE based on the visualisation of occurring hydrogen peroxide production is described. Advantages of this screening method for L-AAOs are the determination of their numbers and approximate molecular weights additionally in one approach.

MeSH terms

Amino Acids / metabolism
Bacteria / enzymology*
Electrophoresis, Polyacrylamide Gel / methods*
Hydrogen Peroxide / metabolism*
L-Amino Acid Oxidase / analysis*
L-Amino Acid Oxidase / isolation & purification
Oxidation-Reduction

Substances

Amino Acids
Hydrogen Peroxide
L-Amino Acid Oxidase