Legionella nagasakiensis sp. nov., isolated from water samples and from a patient with pneumonia

Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):284-288. doi: 10.1099/ijs.0.027193-0. Epub 2011 Mar 11.


A novel Legionella species was identified based on analysis of 16S rRNA and mip (macrophage infectivity potentiator) gene sequences, cellular fatty acids, isoprenoid quinones, biochemical reactions, antigens and quantitative DNA-DNA hybridization. Strain CDC-1796-JAP-E(T) was isolated from well water at the Nagasaki Municipal Medical Center, Japan. Two strains, CDC-3041-AUS-E and CDC-3558-AUS-E, were isolated from water samples during an outbreak of legionellosis in South Australia. Strain CDC-5427-OH-H was isolated from a 66-year-old female patient diagnosed with Legionnaires' disease in the US. Cells from these four strains were gram-negative, non-fluorescent, rod-shaped, and positive for alkaline phosphatase, esterase, leucine arylamidase, catalase, gelatinase, β-lactamase and tyrosine browning assay. Phylogenetic analysis of 16S rRNA and mip genes revealed that the four strains formed a distinct cluster within the genus Legionella. The bacteria contained branched-chain fatty acids and quinones that are typical of members of the genus Legionella. Slide agglutination tests demonstrated no cross-reaction with 52 previously described members of the Legionellaceae. DNA-DNA hybridization studies indicated that DNAs from the four strains were highly related (78-84 %) but they showed 29 % relatedness to Legionella oakridgensis ATCC 33761(T) and less than 10 % to strains of other Legionella species tested. These characterizations suggest that the isolates represent a novel species, for which the name Legionella nagasakiensis sp. nov. is proposed; the type strain is CDC-1796-JAP-E(T) ( = ATCC BAA-1557(T) = JCM 15315(T)).

MeSH terms

  • Aged
  • Bacterial Proteins / genetics
  • Bacterial Typing Techniques
  • DNA, Bacterial / analysis
  • DNA, Ribosomal / analysis
  • Fatty Acids / analysis
  • Female
  • Fresh Water / microbiology*
  • Genes, rRNA
  • Humans
  • Japan / epidemiology
  • Legionella / classification*
  • Legionella / genetics
  • Legionella / isolation & purification*
  • Legionella / physiology
  • Legionellosis / microbiology*
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Peptidylprolyl Isomerase / genetics
  • Phylogeny
  • Pneumonia, Bacterial / microbiology*
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • South Australia / epidemiology
  • Species Specificity
  • United States / epidemiology
  • Water Supply*


  • Bacterial Proteins
  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Quinones
  • RNA, Ribosomal, 16S
  • Peptidylprolyl Isomerase

Associated data

  • GENBANK/EU701006
  • GENBANK/EU701007
  • GENBANK/FJ236837
  • GENBANK/FJ236838
  • GENBANK/FJ236839
  • GENBANK/FJ236840
  • GENBANK/FJ236841
  • GENBANK/FJ236842