PdERECTA, a leucine-rich repeat receptor-like kinase of poplar, confers enhanced water use efficiency in Arabidopsis

Planta. 2011 Aug;234(2):229-41. doi: 10.1007/s00425-011-1389-9. Epub 2011 Mar 12.

Abstract

Water deficiency causes a dramatic reduction in crop production globally. Breeding crop varieties that are more efficient in their water use is one strategy to overcome this predicament. In this study, a member of the LRR-RLKs family, the Populus nigra × (Populus deltoides × Populus nigra) ERECTA (PdERECTA) gene was cloned. To study the biological functions of PdERECTA, transgenic Arabidopsis plants (35S:PdERECTA) that constitutively expressed the PdERECTA gene were constructed. Overexpression of PdERECTA resulted in early seedling establishment, longer primary roots, and larger leaf areas. Notably, transgenic Arabidopsis overexpressing PdERECTA resulted in enhanced long-term water use efficiency (WUEl), as estimated by the analysis of carbon isotopic discrimination. The WUEl results were supported by the physiological and anatomical results, which included improved photosynthetic rate, decreased transpiration rate, and stomatal density. The transgenic lines have significantly more dry-biomass as compared to the wild type. Since the overexpression of PdERECTA can strongly enhance the water use efficiency in transgenic Arabidopsis plants, PdERECTA could potentially be used in transgenic breeding to improve the water use efficiency.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Arabidopsis / metabolism
  • Arabidopsis / physiology*
  • Gene Expression Regulation, Plant
  • Genotype
  • Molecular Sequence Data
  • Mutation
  • Phenotype
  • Photosynthesis
  • Phylogeny
  • Plant Leaves / genetics
  • Plant Leaves / metabolism
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism
  • Plant Roots / genetics
  • Plant Roots / metabolism
  • Plant Shoots / genetics
  • Plant Shoots / metabolism
  • Plant Stomata / physiology
  • Plant Transpiration
  • Plants, Genetically Modified / genetics
  • Plants, Genetically Modified / metabolism
  • Plants, Genetically Modified / physiology
  • Populus / enzymology
  • Populus / genetics*
  • Protein-Serine-Threonine Kinases / chemistry
  • Protein-Serine-Threonine Kinases / genetics*
  • Protein-Serine-Threonine Kinases / metabolism
  • Receptor Protein-Tyrosine Kinases / chemistry
  • Receptor Protein-Tyrosine Kinases / genetics*
  • Receptor Protein-Tyrosine Kinases / metabolism
  • Receptors, Cell Surface / chemistry
  • Receptors, Cell Surface / genetics*
  • Receptors, Cell Surface / metabolism
  • Seedlings / genetics
  • Seedlings / metabolism
  • Sequence Alignment
  • Water / metabolism

Substances

  • Plant Proteins
  • Receptors, Cell Surface
  • Water
  • Receptor Protein-Tyrosine Kinases
  • Protein-Serine-Threonine Kinases
  • leucine-rich repeat receptor-like protein kinase, Populus