Ordered progression of stage-specific miRNA profiles in the mouse B2 B-cell lineage

Blood. 2011 May 19;117(20):5340-9. doi: 10.1182/blood-2010-10-316034. Epub 2011 Mar 14.


Micro-RNAs (miRNAs) have been recognized as critical regulators of gene expression, and deregulation of miRNA expression has been implicated in a wide spectrum of diseases. To provide a framework for the role of miRNAs in B-cell development and malignancy, we deep-sequenced miRNAs from B1 cells and 10 developmental stages that can be identified within the mouse B2 B-cell lineage. The expression profiles of the 232 known miRNAs that are expressed during B-cell development display stage-specific induction patterns, yet hierarchical clustering analysis showed relationships that are in full agreement with the model of the B2 B-cell developmental pathway. Analysis of exemplary miRNA expression profiles (miR-150, miR-146a, miR-155, miR-181) confirmed that our data are in agreement with previous results. The high resolution of the expression data allowed for the identification of the sequential expression of oncomir-1/miR-17-92 and its paralogs miR-106a-363 and miR-106b-25 in subsequent developmental stages in the BM. Further, we have identified and validated 45 novel miRNAs and 6 novel miRNA candidates expressed in developing B cells.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Animals
  • B-Lymphocyte Subsets / classification
  • B-Lymphocyte Subsets / cytology*
  • B-Lymphocyte Subsets / metabolism*
  • Cell Differentiation / genetics
  • Cell Lineage / genetics*
  • Cell Transformation, Neoplastic / genetics
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Mice
  • Mice, Inbred C57BL
  • MicroRNAs / genetics*
  • MicroRNAs / metabolism*
  • RNA, Untranslated / genetics
  • RNA, Untranslated / metabolism


  • MicroRNAs
  • RNA, Untranslated