Effects of Dietary Zinc on Gene Expression of Antioxidant Enzymes and Heat Shock Proteins in Hepatopancreas of Abalone Haliotis Discus Hannai

Comp Biochem Physiol C Toxicol Pharmacol. 2011 Jun;154(1):1-6. doi: 10.1016/j.cbpc.2011.03.003. Epub 2011 Mar 23.

Abstract

The expression patterns of different genes encoding antioxidant enzymes and heat shock proteins were investigated, in present study, by real-time quantitative PCR in the hepatopancreas of abalone Haliotis discus hannai fed with different levels of dietary zinc (6.69, 33.8, 710.6 and 3462.5 mg/kg) for 20 weeks. The antioxidant enzymes include Cu/Zn-superoxide dismutase (Cu/Zn-SOD), Mn-superoxide dismutase (Mn-SOD), catalase (CAT), mu-glutathione-s-transferase (mu-GST) and thioredoxin peroxidase (TPx). The results showed that the mRNA expression of these antioxidant enzymes increased and reached the maximum at the dietary zinc level of 33.8 mg/kg, and then dropped progressively. Expression levels of the heat shock proteins (HSP26, HSP70 and HSP90) firstly increased at 33.8 mg/kg dietary Zn level, and reached to the maximum at 710.6 mg/kg, then dropped at 3462.5 mg/kg (p<0.05). Excessive dietary Zn (710.6 and 3462.5 mg/kg) significantly increases the Zn content and significantly decreases the total antioxidant capacity (T-AOC) in hepatopancreas (p<0.05). These findings showed that dietary Zn (33.8 mg/kg) could highly trigger the expression levels of antioxidant enzymes and heat shock proteins, but excessive dietary Zn (710.6 and 3462.5 mg/kg) induces a high oxidative stress in abalone.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antioxidants / metabolism
  • Catalase / genetics
  • Dose-Response Relationship, Drug
  • Enzymes / genetics*
  • Gastropoda / enzymology
  • Gastropoda / genetics*
  • Gastropoda / metabolism
  • Gene Expression / drug effects*
  • Glutathione Transferase / genetics
  • HSP70 Heat-Shock Proteins / genetics
  • HSP90 Heat-Shock Proteins / genetics
  • Heat-Shock Proteins / genetics*
  • Hepatopancreas / enzymology
  • Hepatopancreas / metabolism*
  • Oxidative Stress / drug effects
  • Peroxiredoxins / genetics
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Superoxide Dismutase / genetics
  • Zinc / administration & dosage
  • Zinc / pharmacology*
  • Zinc / toxicity

Substances

  • Antioxidants
  • Enzymes
  • HSP70 Heat-Shock Proteins
  • HSP90 Heat-Shock Proteins
  • Heat-Shock Proteins
  • RNA, Messenger
  • Peroxiredoxins
  • Catalase
  • Superoxide Dismutase
  • Glutathione Transferase
  • Zinc