Effect of interleukin 1 (IL-1) on the levels of cytochrome P-450 involving IL-1 receptor on the isolated hepatocytes of rat

K Sujita; F Okuno; Y Tanaka; Y Hirano; Y Inamoto; S Eto; M Arai

doi:10.1016/0006-291x(90)91158-o

Effect of interleukin 1 (IL-1) on the levels of cytochrome P-450 involving IL-1 receptor on the isolated hepatocytes of rat

Biochem Biophys Res Commun. 1990 May 16;168(3):1217-22. doi: 10.1016/0006-291x(90)91158-o.

Authors

K Sujita¹, F Okuno, Y Tanaka, Y Hirano, Y Inamoto, S Eto, M Arai

Affiliation

¹ First Department of Internal Medicine, University of Occupational and Environmental Health, School of Medicine, Kitakyushu, Japan.

PMID: 2140678
DOI: 10.1016/0006-291x(90)91158-o

Abstract

In the present study, the expression of IL-1 receptor (IL-1 R) on the primary cultured hepatocytes was determined and the effect of IL-1 on the intracellular amount of cytochrome P-450 was investigated. The kinetics of IL-1R on the hepatocytes was studied by [125I]-IL-1 alpha and revealed that 5,000 molecules of IL-1 bound to a hepatocyte with a Kd of 4.0 x 10(-9) M. Incubation of the hepatocytes with IL-1 for 18 hrs decreased the contents of cytochrome P-450 in a dose-dependent manner. These findings suggest that IL-1 potentially depresses the drug metabolism involving IL-1R which is expressed on hepatocytes.

MeSH terms

Animals
Cells, Cultured
Cytochrome P-450 Enzyme System / metabolism*
Interleukin-1 / metabolism
Interleukin-1 / physiology*
Kinetics
Liver / enzymology
Liver / metabolism*
Male
Rats
Rats, Inbred Strains
Receptors, Immunologic / biosynthesis*
Receptors, Immunologic / metabolism
Receptors, Interleukin-1
Recombinant Proteins

Substances

Interleukin-1
Receptors, Immunologic
Receptors, Interleukin-1
Recombinant Proteins
Cytochrome P-450 Enzyme System