Effect of interleukin 1 (IL-1) on the levels of cytochrome P-450 involving IL-1 receptor on the isolated hepatocytes of rat

Biochem Biophys Res Commun. 1990 May 16;168(3):1217-22. doi: 10.1016/0006-291x(90)91158-o.


In the present study, the expression of IL-1 receptor (IL-1 R) on the primary cultured hepatocytes was determined and the effect of IL-1 on the intracellular amount of cytochrome P-450 was investigated. The kinetics of IL-1R on the hepatocytes was studied by [125I]-IL-1 alpha and revealed that 5,000 molecules of IL-1 bound to a hepatocyte with a Kd of 4.0 x 10(-9) M. Incubation of the hepatocytes with IL-1 for 18 hrs decreased the contents of cytochrome P-450 in a dose-dependent manner. These findings suggest that IL-1 potentially depresses the drug metabolism involving IL-1R which is expressed on hepatocytes.

MeSH terms

  • Animals
  • Cells, Cultured
  • Cytochrome P-450 Enzyme System / metabolism*
  • Interleukin-1 / metabolism
  • Interleukin-1 / physiology*
  • Kinetics
  • Liver / enzymology
  • Liver / metabolism*
  • Male
  • Rats
  • Rats, Inbred Strains
  • Receptors, Immunologic / biosynthesis*
  • Receptors, Immunologic / metabolism
  • Receptors, Interleukin-1
  • Recombinant Proteins


  • Interleukin-1
  • Receptors, Immunologic
  • Receptors, Interleukin-1
  • Recombinant Proteins
  • Cytochrome P-450 Enzyme System