Association of serum 8-hydroxy-2'-deoxyguanosine levels with the presence and severity of coronary artery disease

Coron Artery Dis. 2011 Jun;22(4):223-7. doi: 10.1097/MCA.0b013e328344b615.


Objective: Oxidative stress is involved in the pathogenesis of atherosclerosis. 8-Hydroxy-2'-deoxyguanosine (8-OHdG), an oxidative product of DNA, is a sensitive biomarker to reflect oxidative stress status in vivo. However, the circulating 8-OHdG levels in patients with coronary artery disease (CAD) have not yet been elucidated. The purpose of this study is to investigate whether serum 8-OHdG levels are associated with the presence and severity of CAD.

Methods: We measured serum 8-OHdG levels by enzyme-linked immunosorbent assay in 127 patients with suspected CAD who underwent coronary angiography. The severity of CAD was assessed by the number of diseased vessels and Gensini score.

Results: The serum 8-OHdG levels in patients with CAD were significantly higher than those in patients with normal coronary arteries [median (interquartile range), 0.41 (0.30-0.57) ng/ml versus 0.32 (0.25-0.43) ng/ml, P = 0.001]. Log (8-OHdG) levels increased with the number of diseased vessels (P = 0.002) and significantly correlated with Gensini score (r = 0.379, P = 0.001). The multivariable logistic regression analysis showed that serum 8-OHdG was independently associated with the presence of CAD (odds ratio, 1.318; P = 0.027).

Conclusion: In conclusion, the serum 8-OHdG levels are increased in patients with CAD and are associated with the severity of coronary artery stenosis. 8-OHdG might serve as an independent factor for predicting CAD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Aged
  • Biomarkers / blood
  • Coronary Angiography
  • Coronary Artery Disease / blood*
  • Coronary Artery Disease / physiopathology
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / blood
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Male
  • Middle Aged
  • Oxidative Stress


  • Biomarkers
  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine