Objective: Oxidative stress is involved in the pathogenesis of atherosclerosis. 8-Hydroxy-2'-deoxyguanosine (8-OHdG), an oxidative product of DNA, is a sensitive biomarker to reflect oxidative stress status in vivo. However, the circulating 8-OHdG levels in patients with coronary artery disease (CAD) have not yet been elucidated. The purpose of this study is to investigate whether serum 8-OHdG levels are associated with the presence and severity of CAD.
Methods: We measured serum 8-OHdG levels by enzyme-linked immunosorbent assay in 127 patients with suspected CAD who underwent coronary angiography. The severity of CAD was assessed by the number of diseased vessels and Gensini score.
Results: The serum 8-OHdG levels in patients with CAD were significantly higher than those in patients with normal coronary arteries [median (interquartile range), 0.41 (0.30-0.57) ng/ml versus 0.32 (0.25-0.43) ng/ml, P = 0.001]. Log (8-OHdG) levels increased with the number of diseased vessels (P = 0.002) and significantly correlated with Gensini score (r = 0.379, P = 0.001). The multivariable logistic regression analysis showed that serum 8-OHdG was independently associated with the presence of CAD (odds ratio, 1.318; P = 0.027).
Conclusion: In conclusion, the serum 8-OHdG levels are increased in patients with CAD and are associated with the severity of coronary artery stenosis. 8-OHdG might serve as an independent factor for predicting CAD.