Drosophila neuroblasts retain the daughter centrosome

Nat Commun. 2011;2:243. doi: 10.1038/ncomms1245.

Abstract

During asymmetric mitosis, both in male Drosophila germline stem cells and in mouse embryo neural progenitors, the mother centrosome is retained by the self-renewed cell; hence suggesting that mother centrosome inheritance might contribute to stemness. We test this hypothesis in Drosophila neuroblasts (NBs) tracing photo converted centrioles and a daughter-centriole-specific marker generated by cloning the Drosophila homologue of human Centrobin. Here we show that upon asymmetric mitosis, the mother centrosome is inherited by the differentiating daughter cell. Our results demonstrate maturation-dependent centrosome fate in Drosophila NBs and that the stemness properties of these cells are not linked to mother centrosome inheritance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain / cytology
  • Brain / metabolism
  • Cell Culture Techniques
  • Cell Cycle Proteins / genetics
  • Cell Cycle Proteins / metabolism*
  • Cell Differentiation
  • Centrioles / genetics*
  • Centrioles / metabolism
  • Centrioles / ultrastructure
  • Cloning, Molecular
  • Drosophila / cytology
  • Drosophila / genetics
  • Drosophila / metabolism
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Female
  • Gene Expression
  • Humans
  • Inheritance Patterns / genetics
  • Larva / cytology
  • Larva / genetics
  • Larva / metabolism
  • Male
  • Microscopy, Confocal
  • Mitosis
  • Neural Stem Cells / cytology*
  • Neural Stem Cells / metabolism
  • Photoaffinity Labels / analysis
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism*

Substances

  • CNTROB protein, human
  • Cell Cycle Proteins
  • Drosophila Proteins
  • Photoaffinity Labels
  • Recombinant Proteins