Post-translational processing of the glycoproteins of lymphocytic choriomeningitis virus

Virology. 1990 Jul;177(1):175-83. doi: 10.1016/0042-6822(90)90471-3.


Intracellular events in the synthesis, glycosylation, and transport of the lymphocytic choriomeningitis virus (LCMV) glycoproteins have been examined. We have shown by N-glycanase digestion that LCMV strain Arm-4 bears five oligosaccharides on GP-1 and two on GP-2. By pulse-chase labeling experiments in the presence of drugs which inhibit N-linked oligosaccharide addition and processing we demonstrate that addition of high mannose precursor oligosaccharides is necessary for transport and cleavage of the viral GP-C glycoprotein. Moreover, in the presence of tunicamycin which inhibits en bloc addition of these mannose-rich side chains, virus budding was substantially decreased and infectious virions were reduced by more than 1000-fold in the supernatant medium. Incubation in the presence of castantospermine, which permits addition of oligomannosyl-rich chains but blocks further processing, restored transport and cleavage of GP-C and maturation of virions. Finally, by temperature block experiments we have determined that maturation of GP-C oligosaccharides to an endoglycosidase H resistant form precedes cleavage to GP-1 and GP-2. The latter process is most likely to occur in the Golgi or post-Golgi compartment.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 1-Deoxynojirimycin
  • Alkaloids / pharmacology
  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Cell Line
  • Glucosamine / analogs & derivatives
  • Glucosamine / pharmacology
  • Glycoproteins / biosynthesis
  • Glycoproteins / genetics*
  • Glycoside Hydrolases / antagonists & inhibitors
  • Glycosylation
  • Golgi Apparatus / metabolism
  • Indolizines*
  • Kinetics
  • Lymphocytic choriomeningitis virus / drug effects
  • Lymphocytic choriomeningitis virus / genetics*
  • Lymphocytic choriomeningitis virus / ultrastructure
  • Protein Processing, Post-Translational*
  • Temperature
  • Tunicamycin / pharmacology


  • Alkaloids
  • Anti-Bacterial Agents
  • Glycoproteins
  • Indolizines
  • Tunicamycin
  • 1-Deoxynojirimycin
  • Glycoside Hydrolases
  • Glucosamine
  • castanospermine