Noninvasive imaging of oral neoplasia with a high-resolution fiber-optic microendoscope

Head Neck. 2012 Mar;34(3):305-12. doi: 10.1002/hed.21735. Epub 2011 Mar 16.


Background: The purpose of this study was to evaluate the ability of high-resolution microendoscopy to image and quantify changes in cellular and architectural features seen in early oral neoplasia in vivo.

Methods: A high-resolution microendoscope (HRME) was used to image intact, resected oral squamous carcinoma specimens. HRME images were reviewed and classified as non-neoplastic or neoplastic by expert clinicians. An algorithm based on quantitative morphologic features was also used to classify each image. Results were compared to the histopathologic diagnosis.

Results: HRME images were obtained from 141 sites in resected specimens from 13 patients. Subjective image interpretation yielded sensitivity and specificity of 85% to 90% and 80% to 85%, respectively, whereas the objective classification algorithm achieved sensitivity and specificity of 81% and 77%, respectively.

Conclusion: High-resolution microendoscopy of intact oral mucosa can provide images with sufficient detail to classify oral lesions by both subjective image interpretation and objective image analysis.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Carcinoma, Squamous Cell / pathology*
  • Endoscopes*
  • Endoscopy
  • Fiber Optic Technology / instrumentation*
  • Humans
  • Image Enhancement / instrumentation*
  • Microscopy / instrumentation
  • Mouth Neoplasms / pathology*
  • Precancerous Conditions / pathology*
  • Sensitivity and Specificity